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Transfection of wild-type and mutant cyclin E proteins into NIH 3T3 cells. NIH 3T3 cells were transiently transfected with increasing amounts of myc-tagged wild-type or mutant cyclin E. (A) Percentage of cells in G1 phase of the cell cycle versus amount of transfected plasmid. (B) Cyclin E protein expression and associated histone H1 kinase activity from cells depicted in A.
