Table 1.
Overexpression phenotypes of cyclin E alanine scanning mutants in NIH 3T3 cells
| Amino acids | Histone H1 kinase | G1 acceleration |
|---|---|---|
| Wild type | +++ | +++ |
| 12–14 (RSR) | +++ | ++ |
| 15–17 (KRK) | +++ | +++ |
| 26–30 (DPDEE) | +++ | +++ |
| 33–36 (KIDR) | +++ | +++ |
| 39–40 (RD) | ++ | ++ |
| 64–67 (DKED) | − | − |
| 68–70 (DDR) | +++ | +++ |
| 78–80 (KPR) | +++ | +++ |
| 98–100 (REE) | +++ | +/− |
| 108–110 (KEK) | − | − |
| 114–117 (RDQH) | +++ | +++ |
| 246–249 (DLHE) | +++ | +/− |
| 273–278 (DVDCLE) | ++ | − |
| 324–325 (RE) | ++ | ++ |
| 330–335 (KLKHFR) | +++ | ++ |
| 339–341 (DED) | +++ | ++ |
| 348–350 (HRD) | +++ | ++ |
| 353–357 (DLLDK) | +++ | +/− |
| 359–362 (RAKK) | +++ | ++ |