Figure 1.

SDS/PAGE of the purified pBQ-dC repair enzyme (left lane) and protein size markers (right lane). Sixty-seven nanograms of the peak active fraction from the final purification step (Superdex 75) was loaded on the 12% gel, which was subsequently stained using a silver staining kit (Pharmacia). The calculated molecular mass of the pBQ-dC enzyme indicated by the arrow is 38.1 kDa. The molecular weight standards were BSA (66 kDa), ovalbumin (45 kDa), carbonic anhydrase (29 kDa), trypsinogen (24 kDa), and trypsin inhibitor (20.1 kDa).