Skip to main content
NCBI home page
Canadian Medical Association Journal logoLink to Canadian Medical Association Journal
. 1972 Sep 23;107(6):509–515.

In vitro study of macrophages from patients with sarcoidosis

V Kurti, E Mankiewicz
PMCID: PMC1940919  PMID: 5057007

Abstract

Cultures of leukocytes from patients with sarcoidosis were stimulated with Kveim antigen or PPD-S and the macrophage reactions were compared with those obtained under similar conditions with leukocyte cultures from healthy tuberculin reactors. The non-stimulated control cultures of Kveim-positive sarcoid patients showed signs of macrophage activation, namely an increase in cytoplasmic pyroninophilia in small and enlarged macrophages, together with a higher than normal rate of lymphoblast transformation. In the presence of Kveim antigen this activation was more marked, even in cases where the blast response to the antigen was of low magnitude. Stimulation with tuberculoproteins produced a slight activation of macrophages only in cultures from Kveim reactors who had also shown positive tuberculin reactions.

These observations suggest that the study of lymphocyte transformation together with that of macrophage activation will improve the immunocytological assessment of sarcoidosis.

Full text

PDF
509

Images in this article

511FIG. 1
on p.511
511FIG. 2
on p.511
511FIG. 3
on p.511
512FIG. 4
on p.512
512FIG. 5
on p.512
512FIG. 6
on p.512
512FIG. 7
on p.512
512FIG. 8
on p.512
512FIG. 9
on p.512

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berthrong M. The macrophage in tuberculosis. Bibl Tuberc. 1970;26:1–27. [PubMed] [Google Scholar]
  2. COHN Z. A., BENSON B. THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965 Jan 1;121:153–170. doi: 10.1084/jem.121.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHN Z. A., BENSON B. THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. II. THE INFLUENCE OF SERUM ON GRANULE FORMATION, HYDROLASE PRODUCTION, AND PINOCYTOSIS. J Exp Med. 1965 May 1;121:835–848. doi: 10.1084/jem.121.5.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FISHMAN M. Antibody formation in tissue culture. Nature. 1959 Apr 25;183(4669):1200–1201. doi: 10.1038/1831200a0. [DOI] [PubMed] [Google Scholar]
  5. HIRSCHHORN K., SCHREIBMAN R. R., BACH F. H., SILTZBACH L. E. IN-VITRO STUDIES OF LYMPHOCYTES FROM PATIENTS WITH SARCOIDOSIS AND LYMPHOPROLIFERATIVE DISEASES. Lancet. 1964 Oct 17;2(7364):842–843. doi: 10.1016/s0140-6736(64)90691-9. [DOI] [PubMed] [Google Scholar]
  6. Lewis M. R. The Formation of Macrophages, Epithelioid Cells and Giant Cells from Leucocytes in Incubated Blood. Am J Pathol. 1925 Jan;1(1):91–100.1. [PMC free article] [PubMed] [Google Scholar]
  7. Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mankiewicz E., Kurti V., Béland J. Diagnostic procedures in sarcoidosis. Can Med Assoc J. 1971 Apr 17;104(8):684–690. [PMC free article] [PubMed] [Google Scholar]
  9. Oppenheim J. J. Immunological relevance of antigen and antigen antibody complex induced lymphocyte transformation. Ann Allergy. 1969 Jul;27(7):305–315. [PubMed] [Google Scholar]
  10. Rechardt L., Mustakallio K. K. Macrophage response to epidermal abrasion in sarcoidosis. Its relation to the patient's Kveim reactivity. Acta Pathol Microbiol Scand. 1965;65(4):521–527. doi: 10.1111/apm.1965.65.4.521. [DOI] [PubMed] [Google Scholar]
  11. Sanford K. K., Barker B. E., Parshad R., Westfall B. B., Woods M. W., Jackson J. L., King D. R., Peppers E. V. Neoplastic conversion in vitro of mouse cells: cytologic, chromosomal, enzymatic, glycolytic, and growth properties. J Natl Cancer Inst. 1970 Dec;45(6):1071–1096. [PubMed] [Google Scholar]
  12. Schmidt M., Havemann K., Kessler G. F., Malchow H. Untersuchungen zum Ribonukleinsäure (RNS)-Stoffwechsel von Monocyten in vitro bei Gesunden und Patienten mit Sarcoidose. Z Immunitatsforsch Allerg Klin Immunol. 1970 Feb;139(2):195–208. [PubMed] [Google Scholar]
  13. Sutton J. S. Ultrastructural aspects of in vitro development of monocytes into macrophages, epithelioid cells, and multinucleated giant cells. Natl Cancer Inst Monogr. 1967 Sep;26:71–141. [PubMed] [Google Scholar]
  14. Sutton J. S., Weiss L. Transformation of monocytes in tissue culture into macrophages, epithelioid cells, and multinucleated giant cells. An electron microscope study. J Cell Biol. 1966 Feb;28(2):303–332. doi: 10.1083/jcb.28.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. UEHLINGER E. A. The morbid anatomy of sarcoidosis. Am Rev Respir Dis. 1961 Nov;84(5):6–13. doi: 10.1164/arrd.1961.84.5P2.6. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Medical Association Journal are provided here courtesy of Canadian Medical Association

RESOURCES