Figure 2.

Relationship between the different acyl-CoA oxidases visualized in Western blots. Aliquots of bacterial lysate (20 μg of protein), containing thioredoxin–hBRCACox fusion protein (theoretical molecular mass, 91 kDa), were analyzed for cross-reactivity with different antisera. (a) Anti-thioredoxin. (b) Anti-hBRCACox. (c) Anti-rTHCCox. (d) Anti-SKL. (e) Anti-rPALMCox (B subunit). (f) Anti-rPALMCox (C subunit). (g) Anti-rPRISCox. Lanes 1 and 2 represent a noninduced culture and an induced culture, respectively. Migration of molecular weight markers (expressed in kDa) is indicated at the left.