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. 2007 Jul 17;104(30):12294–12299. doi: 10.1073/pnas.0701817104

Fig. 2.

Fig. 2.

Solubilization of monogeranylgeranylated Rab7 by the β-subunit of RabGGTase and its interaction analysis with REP and GDI. (A) Gel filtration chromatography of Rab7-G:βGGT on a Superdex 200 column (Upper) and SDS/PAGE analysis of resulting fractions (Lower). (B) MALDI-TOF analysis of Rab7-G:βGGT complex (Mcalc = 23,660 Da for Rab7-G). (C and D) Titration of REP (C) or GDI (D) to a mixture of 10 nM Rab7-G:βGGT and 10 nM Rab7-NF. The data were fitted by numerical simulation and fitting to a competitive binding model to give Kd values for the interaction of Rab7-G with REP or GDI. (E and F) Titration of REP (E) or GDI (F) to a mixture of 25 nM Rab7-NF and 25 nM Rab7d-GG:BSA (λex/em:479/525 nm). The data were fitted to a competitive binding model to give Kd values of Rab7d-GG for REP and GDI, where Kd values of 0.22 nM and 21 nM of Rab7-NF for REP and GDI, respectively, were fixed.