Fig. 6.
Changes in protein subcellular distribution in response to DNA damage. (A and C) WS1 (A) or MCF7 (C) cells were either left untreated or treated with 300 ng/ml NCS for 2 and 5 h or NCS plus proteasome inhibitors for 5 h. (E) U2OS cells were either left untreated or treated with doxycycline and/or NCS as described in Fig. 4. Lysates from the nuclear fraction and the cytoplasmic fraction representing the same numbers of cells were analyzed by the Western blotting as described in Fig. 1. (B, D, and F) The charts representing the estimated protein molecules in the nuclear fraction. The y axis represents the percentage of each protein in the nuclear fraction. Numbers shown in the box represent the protein molecules × 103 per cell in the nuclear fraction.