Table 1.
Strains and plasmids
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| E. coli | ||
| RP437 | Wild type for motility | (11) |
| RP8611 | Δtsr, Δ(tar-tap), Δtrg | (12) |
| RP9352 | Δtsr, Δ(tar-tap), Δtrg, ΔcheZ | (12) |
| RP2867 | Δ(tap-cheB) | (13) |
| RP9535 | ΔcheA | (J. S. Parkinson, Univ. of Utah, Salt Lake City) |
| RP4782 | fliM182 | (14) |
| RP4783 | fliM183 | (14) |
| HCB429 | Δtsr, Δ(tar-tap), Δtrg | (15) |
| S. typhimurium | ||
| SJW1103 | Wild type for motility | (16) |
| KK2051 | cheA∷Tn10 | (17) |
| MY107 | fliG N72Y | (18) |
| SJW2323 | fliG V135F | (18) |
| Plasmids* | ||
| pJC3-Tsr | tsr+ | (J. S. Parkinson) |
| pJC3-Tsr(R64C) | tsr (190 C → T) | This work |
| pJC3-Tsr(T156P) | tsr (466 A → C) | This work |
| pJC3-Tsr(A413V) | tsr (1238 C → T) | This work |
| pNT201-Tar | tar+ | (19) |
| pNT201-Tar(R69K) | tar (205-207 CGT → AAA) | This work |
| pNT201-Tar(T154I) | tar (461 C → T) | This work |
| pMK76-Tar(M76K) | tar (227 T → A) | (20) |
| pAL1-Trg | trg+ | (21) |
| pCG1-Tap | tap+ | This work |
| pPA56 | Cytoplasmic Tsr domain | (ref. 22; see Methods) |
| pMS103 | Cytoplasmic Tar domain | (ref. 23; see Methods) |
| pMS105 | Cytoplasmic Tar domain | (ref. 23; see Methods) |
*
Genes on most plasmids are expressed from the tac promoter; pMS plasmids use the trc promoter, whereas pMK76 uses the natural Tar promoter. The numbers in parentheses under “relevant genotype” of plasmids refer to the nucleotide positions, starting at the first nucleotide of the ATG codon.