Fig. 1.

Gelatin and casein zymography of tissue extracts from leaking bleb tissue. Equal protein aliquots of bleb tissue extracts and normal conjunctival extracts were electrophoresed on SDS-polyacrylamide gels containing 0·1% gelatin or casein. Bleb leak fluid and aqueous humour samples were also analysed. Mixed MMPs contained in conditioned medium from rabbit corneal fibroblasts treated with phorbol myristate acetate served as positive control standards. The high molecular weight caseinase is indicated with an arrow. Rabbit gelA is slightly lower in molecular weight than human gelA. GelB was specific for bleb tissue.