Fluorescence microscopy of 2 μM 4-Di-1-ASP alone (A), 1 μM MitoTracker Deep Red 633 alone (B), and merged (C). Uptake of these compounds in BeWo cells grown on a microscope slide was at 37°C for 30 minutes. Detection settings for 4-Di-1-ASP: excitation, 477 nm; emission, 557 nm. For MitoTracker Deep Red 633: excitation, 633 nm; emission, 789 nm.