Figure 1.

Cdc13p is a ss TG_1–3 binding protein. ³²P-labeled TG22, a 22-base oligonucleotide of ss TG_1–3 DNA (Table 1), was used in gel mobility-shift assays using total extracts from either bacteria (Ec) or yeast (Ysc). All gel mobility-shift assays reported here were done in the presence of ss poly(dI·dC). E. coli extracts were prepared from cells expressing Cdc13p or carrying the vector alone. Yeast extracts were prepared from a CDC13 strain carrying vector alone or pTHA-CDC13 for overexpression of Cdc13p. The arrows point to a TG22-protein complex that was seen only in E. coli cells expressing Cdc13p or yeast cells overexpressing Cdc13p. For both panels, the first lane had no extract. (A) The amount of extract used is indicated. (B) Two micrograms of extract was used.