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. Author manuscript; available in PMC: 2007 Aug 8.
Published in final edited form as: J Mol Biol. 2006 Dec 1;366(5):1459–1474. doi: 10.1016/j.jmb.2006.11.070

Figure 6.

Figure 6

Thermal denaturation of full-length and N-terminally truncated versions of poliovirus 3Dpol. (a) Denaturation profiles of native 3Dpol (●), 3Dpol-Δ22 (×), and 3Dpol-Δ68 (□) obtained by CD spectroscopy at a wavelength of 222 nm. (b) Slope analysis plot (Δθ vs. T) of data in panel (a) where the maxima indicate the thermal transition temperatures of the various proteins (Tx - summarized in Table 2) (c) Far UV CD spectra of full-length 3Dpol(○) obtained at 10°C and the 3Dpol-Δ68 mutant at 10°C (●), 50°C (◆), 60°C (□), and 90°C (+).