Table 1.
Summary of crystallographic data
| Complex | 3Dpol+UTP+Mg2+ | 3Dpol+CTP+Mg2+ | 3Dpol+GTP+Mg2+ | 3Dpol+ATP+Mg2+ | 3Dpol+UTP+Mn2+ | 3Dpol+CTP+Mn2+ | 3Dpol+GTP+Mn2+ |
|---|---|---|---|---|---|---|---|
| PDB Code | 2IM2 | 2IM0 | 1RA71 | 2ILY | 2IM3 | 2IM1 | 2ILZ |
| Space Group | P65 | P65 | P65 | P65 | P65 | P65 | P65 |
| Unit Cell (Å) | a=b=129.0 c=113.1 | a=b=127.7 c=113.0 | a=b= 128.0 c= 113.3 | a=b=128.5 c=112.9 | a=b=128.1 c=112.8 | a=b=128.9 c=113.0 | a=b=128.2 c=112.6 |
| X-Ray Source | Cu-Kα 1.54 Å | ALS 4.4.2 0.98 Å | Cu-Kα 1.54 Å | Cu-Kα 1.54 Å | Cu-Kα 1.54 Å | Cu-Kα 1.54 Å | Cu-Kα 1.54 Å |
| Resolution Limits (Å)2 | 30 - 2.35 (2.43-2.45) | 30 - 2.25 (2.33-2.25) | 30 - 2.35 (2.43-2.35) | 30 - 2.6 (2.69-2.60) | 30 - 2.6 (2.69-2.60) | 30 - 2.5 (2.59-2.50) | 30 - 2.5 (2.59-2.50) |
| C α RMSD vs. apo | 0.26 Å | 0.18 Å | 0.23 Å | 0.23 Å | 0.26 Å | 0.28 Å | 0.27 Å |
| Reflections | |||||||
| Total Collected | 313203 | 372805 | 215638 | 168973 | 276952 | 302975 | 136298 |
| Unique | 43793 | 49540 | 43126 | 31801 | 32149 | 36470 | 36244 |
| Redundancy | 7.2 (7.1) | 7.5 (6.8) | 5 (3.9) | 5.31 (5.02) | 8.6 (8.5) | 8.3 (8.3) | 3.8 (3.7) |
| I/σ | 16.6 (4.6) | 13.1 (3.6) | 16.2 (2.1) | 11.9 (3.7) | 15.6 (4.7) | 15.5 (4.7) | 9.9 (2.2) |
| Completeness (%) | 98.5 (97.7) | 99.7 (99.5) | 98.6 (96.2) | 97.6 (96.3) | 99.3 (98.8) | 98.8 (98.5) | 99.8 (100) |
| Rmerge (%) | 7.7 (43.9) | 8.5 (46.2) | 8.2 (55.7) | 11.5 (45.8) | 9.0 (45.8) | 8.2 (44.6) | 8.1 (47.6) |
| Refinement | |||||||
| Resolution Range (Å) | 30 - 2.35 | 30 - 2.25 | 30 - 2.35 | 30 - 2.6 | 30 - 2.6 | 30 - 2.5 | 30 - 2.5 |
| R | 22.9 | 23.7 | 22.4 | 22.6 | 23.0 | 23.1 | 23.0 |
| R-free3 | 26.6 | 26.0 | 26.2 | 25.4 | 26.3 | 26.3 | 25.5 |
| Ramachandran statistics4 | |||||||
| Favored | 378 | 373 | 379 | 368 | 363 | 360 | 366 |
| Allowed | 30 | 36 | 30 | 39 | 44 | 48 | 42 |
| Generous | 2 | 1 | 1 | 3 | 4 | 2 | 2 |
| Disfavored | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
1
Previously published (reference 1) and shown for comparison.
2
Data in parentheses are for the highest resolution shell.
3
R-free was computed using 10% of the data assigned randomly.
4
Ramachandran statistics do not include glycine and proline residues.