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Effect of incubation time and serum on ultrasound-mediated transfection of AoSMC by naked DNA and PEI:DNA complexes expressing GFP. The effect of sonication is shown on cells (i) incubated for 4 h after sonication without serum, (ii) not incubated for 4 h after sonication without serum and (iii) incubated for 4 h after sonication with 10% fetal bovine serum. (average ± SEM, n ≥ 3).
