Skip to main content
. 2007 Jul 2;7:79. doi: 10.1186/1471-213X-7-79

Table 1.

In situelectroporation efficiently delivers the construct to the blood islands

Frequency of recovered hematopoietic precursors Number of recovered hematopoietic precursors per YS Number of experiments
Whole OrgD3-YS 1/475 10–11 4
Whole OrgD3-YS electroporated without plasmid 1/453 10 3
OrgD3-YS GFP+cells 1/150 (± 57.62) 0.56 ± 0.18 per 0.5 YS (targeted area) 5

Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay.