Table 2.
Assets and limitation of in situ electroporation and retroviral transduction to target YS blood islands precursors
| In situ electroporation | Retroviral transduction | |||
| Asset | Limitation | Asset | Limitation | |
| 7–7.5 dpc: Mesodermal/pre-hematopoietic precursors | - Transient - Layer specific delivery - Spatial control of delivery |
- Reduced number of precursors available for transduction | - Causes extensive cell death - Refractory to transduction ? |
|
| Application | Only possible method | Unsuitable at this development stage | ||
| From 7.5–8 dpc: Hematopoietic precursors | - Transient - Layer specific delivery - Spatial control of delivery |
- Only a fraction of the YS blood islands is targeted | - Easy - All hematopoietic precursors may be transduced |
- Does not allow layer specific targeting |
| Application | -Transient transgene delivery | - Sustained expression of the transgene | ||
| - Possible layer specific delivery. | - Possible delivery of ShRNA | |||
| - Possible delivery of SiRNA or proteins | ||||
Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay.