Figure 1. Low Oxygen Expression of Tf2 mRNA and Protein Requires Sre1.

(A) Wild-type yeast were cultured in the absence of oxygen for increasing time. Upper panel: total RNA (10 μg) was subjected to northern analysis using a Tf2 probe. Lower panel: cell extracts (40 μg) were analyzed by immunoblotting using antibodies to Tf2 reverse transcriptase (RT), integrase (IN), and the nuclear form of Sre1 [23,27].

(B) Wild-type and sre1Δ yeast were cultured +/− oxygen for 6 h and processed for northern and immunoblot analysis.