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. 2007 Aug 10;3(8):e131. doi: 10.1371/journal.pgen.0030131

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© 2007 Sehgal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Wild-type and sre1Δ yeast carrying Tf2 LTR-lacZ, Tf2–11 LTR-lacZ, or lacZ reporter plasmids were grown +/− oxygen for 6 h and assayed for β-galactosidase activity [26]. Error bars denote one standard deviation among three biological replicates.

(B) Sre1 DNA binding domain (aa 256–366) was incubated with indicated ³²P-labeled DNA probes and subjected to electrophoretic mobility shift assay [26].

(C) Wild-type yeast were grown +/− oxygen for 6 h and subjected to chromatin immunoprecipitation using anti-Sre1 IgG or rabbit IgG. Bound DNA was normalized to wild type + oxygen for each primer pair. The fraction of bound DNA values for the pulldown with anti-Sre1 under aerobic conditions were 0.0027 (Tf2–3), 0.003 (Tf2–4), and 0.017 (Tf2–11). Error bars denote one standard deviation among three experimental replicates.