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. 2007 Aug 10;3(8):e131. doi: 10.1371/journal.pgen.0030131

Figure 5. Endogenous Solo LTRs Function as Oxygen-Dependent Promoters.

Figure 5

(A) Scheme for generating Tf2–6 solo LTR. Recombination between intra-element LTRs yields solo LTR. Arrowheads denote position of RT-PCR primers.

(B) Wild-type and sre1Δ yeast containing Tf2–6 solo LTR were cultured +/− oxygen for 4 h. Transcription downstream of the LTR was quantified by real-time RT-PCR and normalized to values for wild-type cells + oxygen. Error bars denote standard deviation among three real-time RT-PCR replicates.

(C) Wild-type yeast were cultured +/− oxygen for 4 h. Transcription of sequences ∼100 bp downstream of 20 different Tf2 solo LTRs was quantified by real-time RT-PCR as described in Materials and Methods. Fold change in transcription after shifting to low oxygen is shown. Error bars denote standard deviation among three real-time RT-PCR replicates.

(D) Wild-type yeast were cultured +/− oxygen for 6 h. Upper panel: Northern analysis using SPCC11E10.07c and SPAC2E1P3.02c probes. Arrows indicate Tf2 LTR derived transcript. Lower panel: RT-PCR to detect transcripts originating either in Tf2 LTR (A–C) or the full mRNA transcripts (B–C). Bold arrows denote transcription initiation sites. Arrowheads denote primer positions.