Table 2.
Binding of monoclonal antibodies to selected gB mutants.
| Region* | Position of insertion† | Monoclonal antibodies‡ |
|||||
|---|---|---|---|---|---|---|---|
| II-105 | I-84-5 | II-125-4 | I-I-7 | I-144-2 | I-252-4 | ||
| N terminus | P80 | +++ | +++ | +++ | +++ | +++ | ++ |
| Domain I | I185 | +++ | +++ | +++ | +++ | ++ | +++ |
| E187 | +++ | +++ | +++ | +++ | ++ | ++ | |
| A261 | +++ | +++ | +++ | +++ | ++ | ++ | |
| Domain II | D408 | +++ | +++ | +++ | ++ | ++ | ++ |
| Disordered | R470 | +++ | +++ | +++ | +++ | +++ | +++ |
| Linker 2 | I495 | +++ | +++ | +++ | ++ | ++ | +++ |
| T497 | +++ | +++ | +++ | +++ | +++ | +++ | |
| Domain III | D663 | +++ | +++ | +++ | +++ | +++ | ++ |
| T665 | + | + | – | + | – | ++ | |
| V667 | + | – | – | – | – | – | |
| Domain V | I671 | +++ | +++ | ++ | +++ | + | ++ |
| L673 | +++ | + | + | – | – | + | |
| A725 | +++ | +++ | +++ | +++ | +++ | +++ | |
| Membrane-proximal | A730 | +++ | +++ | +++ | +++ | +++ | +++ |
| F732 | +++ | +++ | +++ | +++ | +++ | +++ | |
| M742 | +++ | +++ | +++ | +++ | +++ | +++ | |
| C-terminal tail | T868 | ++ | +++ | + | ++ | + | +++ |
| N886 | ++ | ++ | ++ | ++ | ++ | ++ | |
*From Heldwein et al. (12).
†List includes mutants for which rabbit antiserum R74 binds at >25% of WT gB levels by CELISA.
‡CELISA data were normalized to the reactivity of each mAb to WT gB. The normalized values for each mAb were then compared with values obtained using rabbit antiserum R74 for each gB mutant to determine relative changes in reactivity. The symbols represent >80% (+++), 50–80% (++), 20–50% (+), and <20% (–) reactivity of each mAb compared to R74 values for each gB mutant.