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Southern hybridization analyses of the sopE and orfJ regions of Salmonella spp. Total cell DNA from various S. enterica serovars, digested with HindIII, EcoRV, or both, was probed with a 0.7-kb HindIII/XmnI fragment comprising the 3′ portion of orfJ and the 5′ portion of orfI. Blots were subsequently stripped and reprobed with a DNA fragment corresponding to the coding region of sopE.
