Fig. 2.

siRNA polyconjugates can be used to transfect siRNA in mouse primary hepatocytes. Shown is RT-qPCR analysis of apoB mRNA knockdown in primary hepatocytes. Cells were transfected with the indicated amounts of siRNA by using a commercially available transfection reagent (siQUEST) or with serial dilutions of apoB-1 siRNA polyconjugate. Twenty-four hours after transfection, relative apoB mRNA levels were measured versus GAPDH mRNA levels or versus the amount of input RNA in the RT-qPCR and then normalized to the values in untreated cells (cells alone). Data are shown as mean ± SD.