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. 2007 Jul 24;104(32):12982–12987. doi: 10.1073/pnas.0703778104

Fig. 4.

Fig. 4.

Knockdown of target gene expression in livers of mice after i.v. injection of siRNA polyconjugates. (A) Reduction of apoB mRNA levels in liver after treatment with apoB siRNA polyconjugates. RT-qPCR analyses of liver apoB levels relative to GAPDH mRNA or total input RNA were performed 2 days after injection of apoB-1, apoB-2, or control siRNA polyconjugate (800 μg of polymer, 50 μg of siRNA). Shown are the data normalized to mice receiving saline alone. n = 5, data are shown as mean ± SD. (B) Serum levels of apoB-100 protein are reduced in apoB siRNA polyconjugate-treated mice 2 days after injection. An equal volume of serum from individual mice was pooled for each group (n = 5) and subjected to Western blot analysis. (C) Reduction of ppara mRNA levels in liver after i.v. injection of ppara siRNA polyconjugates. RT-qPCR and Invader analyses of liver ppara mRNA levels relative to GAPDH or ubiquitin mRNA, respectively, 2 days after injection of ppara-1, ppara-2, or control siRNA polyconjugate (800 μg of polymer, 50 μg of siRNA). Shown are the data normalized to mice receiving saline alone. n = 5, data are shown as mean ± SD.