Fig. 2.

Six3 associates with MTA1 in retinal ganglion cells. (A) ChIP, showing Six3 derecruitment from its own promoter in RGC5 cells under MTA1 silencing. (B) EMSA, showing Six3 binding to a synthetic oligo containing ATTA core (lane 3) and a shift with Six3 antibody (lane 4, bracket) and higher-molecular-weight complexes with MTA1 and Six3 (lane 5). Higher protein/DNA complexes are seen in the presence of Six3, MTA1, and Six3 Ab (lane 6). Lane 7 shows no interaction of MTA1 with ATTA DNA oligo.