Figure 2.

Differential inhibition by a unique AAF adduct of ssDNA and forked-DNA replication catalyzed by CHO cell extracts. Sequences of the ssDNA (A) and the forked-DNA template (B) damaged with a unique AAF adduct used in the replication assay. ▪ indicates the position of the AAF adduct. DNA synthesis conditions for ssDNA and forked DNA (C and D) were the same as those described in Fig. 1. Autoradiograph in D was intentionally overexposed to clearly detect TLS on damaged forked DNA. The position of the 15-mer (primer), 31-mer (product of synthesis to the dC base preceding the AAF adduct), and 44-mer (full-size product) are indicated by arrows.