Table 1.
Phenotype of immature dendritic cells (DCs) and lipopolysaccharide (LPS)-activated DCs. DCs were exposed to photoactivable agent 8-methoxypsolaren (8-MOP) and ultraviolet (UV) A (PUVA) treatment as described in Materials and methods. Immature DCs were activated to mature DCs with addition of 10 ng/ml LPS on day 6. Results are presented as mean of mean fluorescence intensity/or percentage ± s.d. of seven independent experiments.
| CD1a | CD14 | CD40 | CD80 | CD83 | HLA-DR | |
|---|---|---|---|---|---|---|
| Immature DCs | ||||||
| Control | 84 ± 10·6% | 2·7 ± 1·4% | 10·6 ± 2·1% | 345·4 ± 57·1 | ||
| PUVA treatment | 81·2 ± 3% | 3·8 ± 2·9% | 9·9 ± 1·9% | 380·8 ± 45* | ||
| Mature DCs | ||||||
| Control | 816·7 ± 52 | 474·7 ± 38·6 | 429·3 ± 44 | 476·5 ± 36·8 | ||
| PUVA treatment | 807 ± 55 | 485·8 ± 35·3 | 421·1 ± 41·4 | 526·2 ± 17·1 | ||
*
Statistical significance (P < 0·01) compared to untreated control DCs. HLA-DR: human leucocyte antigen D-related.