Fig. 4.

β1-Immunoadsorption sera from chronic Chagas heart disease (cChHD) patients. (a) Coraffin immunoadsorption. Enzyme-linked immunosorbent assay reactivity of Coraffin immunoadsorbed sera from cChHD patients with strong anti-β1-adrenergic receptor (AR) reactivity. Results represent the difference between the total reactivity before immunoadsorption (100%), the flow-through fraction reactivity and the captured fraction reactivity of these IgGs. (b) Chronotropic effect of serum p3. The specificity of this effect was studied by subsequent addition of the M2-ChR antagonist atropine and the β1-AR antagonist bisoprolol. Upper panel, immunostaining pattern of serum p3: before immunoadsorption (a), flow-through fraction (b) and captured fraction (c).