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. 2007 Aug;149(2):344–352. doi: 10.1111/j.1365-2249.2007.03421.x

Fig. 1.

Fig. 1

Phenotype and maturation status of cord blood monocyte-derived dendritic cells (DC) upon exposure to selected probiotic bacteria. Immature DC were matured with maturation factors (MF), lipopolysaccharide (LPS) and probiotic bacteria (bacteria : cell ratio 10 : 1) in the presence or absence of MF. After 48 h expression of CD14, CD40, CD80, CD86 and human leucocyte antigen D-related (HLA-DR) was analysed by flow cytometry. (a) Open histograms represent expression by immature DC, solid histograms show the level of expression as a result of treatment. One representative experiment of nine is shown. (b) Mean expression of CD86 and HLA-DR in differently matured DC. The expression of CD86 and HLA-DR of immature DC was set at 100% and the relative expression in other culture conditions was compared to this value. Data represent mean expression ± standard error of the mean (n = 9). Statistical analysis was performed by Mann–Whitney U-test. *P < 0·05 compared to immature DC. Expression of CD86 and HLA-DR of all fully matured DC (MF, LPS, Bifidobacterium bifidum, B. infantis, Lactobacillus salivarius and Lactococcus lactis) was significantly higher (P < 0·01) compared to immature DC.