Fig. 1.

T cell immunoglobulin mucin-1 (TIM-1) antibodies enhance antigen-specific cellular proliferation and interferon (IFN)-γ production. BALB/c mice (four per group) were vaccinated intraperitoneally (i.p.) with 10 µg whole inactivated Beijing influenza virus mixed with 100 µg TIM-1 antibody (α-TIM-1; black triangles), isotype control (IgG2b; open triangles) or phosphate-buffered saline (PBS) (naive; open squares). After 21 days, splenocytes were harvested and cultured in vitro with increasing amounts of whole inactivated Beijing influenza virus for 96 h and analysed for proliferation (a). Supernatants were removed for ELISA analysis of IFN-γ production (b). Data represent the mean ± s.e.m. Statistical significance is denoted by asterisk for values compared to isotype control. *P < 0·05; **P < 0·001.