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. 2006 Jul;145(1):48–55. doi: 10.1111/j.1365-2249.2006.03106.x

Fig. 1.

Fig. 1

(a) Lymphocytes were gated based on forward scatter (FSC) and side scatter (SSC) (gate R1). (b) Regulatory CD4+CD25high+ T cell gate (gate R4) was defined as the CD25+ cells with intensity above that observed in CD8+ cells. (c) CD4+ cells co-expressing intracellular cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) in lymphocyte gate. (d) CD4+ cells co-expressing intracellular CTLA-4 in CD25high+ gate.