The effects of exogenous recombinant interleukin (rIL)-2, rIL-12 and recombinant interferon (rIFN)-α, alone or in combination with bacille Calmette–Guérin (BCG), on IFN-γ production by human peripheral blood mononuclear cells (PBMCs). PBMCs were incubated with the indicated doses of rIL-2 (a), rIL-12 (b) or rIFN-α (c), in the presence or absence of BCG [2·5 × 105 colony-forming units (CFU)/ml], for 3 days and then assayed for IFN-γ production in the conditioned media.