Fig. 4.
Bacille Calmette–Guérin (BCG) dose-dependent interferon (IFN)-γ production by human peripheral blood mononuclear cells (PBMCs) in response to combination of BCG plus exogenous triple cytokines recombinant interleukin (rIL)-2, rIL-12 and rIFN-α. PBMCs were incubated with five different doses of BCG ranging from 2·5 × 103 to 2·5 × 106 colony-forming units (CFU)/ml, in the presence or absence of triple cytokines at a minimal dose, a moderate dose or a high dose for 3 days and then assayed for IFN-γ production in the conditioned media.