Table 1.
Primers used for construction of truncated regions of the EpC1 parent protein.
| Name | Sequence (5′→ 3′) | Use |
|---|---|---|
| EPC3-1 | 5′-GGCTCGAGTCAATCGAAAAGCTTCTCAACAGTT-3′ | DSP for P1 |
| EPC3-2 | 5′-GGCTCGAGTCAATGGTCGTCATCGAGAGGCTC-3′ | DSP for P3, P4 and P8 |
| EPC3-4 | 5′-GGCTCGAGTCACTGTAGTGCGGATTTAAGCTCG-3′ | DSP for P2 and P8 |
| EPC3-10 | 5′-GGCTCGAGTCACTTATCTAAGAAAGCCTTCACATG-3′ | DSP for P5 |
| EPC5-1 | 5′-GGGATCCTGAGTTGGACAAGGACAAGTCG-3′ | USP for P2, P4 and P10 |
| EPC5-2 | 5′-GGGATCCACATGTGAAGGCTTTCTTAGAT-3′ | USP for P6 |
| EPC5-8 | 5′-GGAATTCGCCGAGCTTAAATCCGCACTA-3′ | USP for P3 and P5 |
| EPC5-10 | 5′-GGGATCCGATAAGCTCGACTCCAACAAG-3′ | USP for P7 |
| C1R | 5′ GCCCGAGCAACTTAATCTCGA 3′ | DSP for P6, P7 and P10 |
| S-TAG | 5′ TCGAACGCCAGCACATGGACAG 3′ | USP for P1, P8 and P9 |
Sequences in italics are restriction enzyme cut sites. TCA is a stop codon. DSP, downstream primer; USP, upstream primer.