Fig. 1.

Analysis of four sequential aliquots (1·2 litres each) of fluid flushed from the living donor right liver lobe, immediately prior to transplantation. The samples were subjected to a lymphocyte gate based on light scatter, and analysed by flow cytometry. The data show the change-over from cells that retain some features of blood cells (peripheral blood mononuclear cells) in flush 1 to the typical intrahepatic lymphocytes (IHL) subset distribution of CD56-rich natural killer and CD8⁺ rather than CD4⁺ T cells by flush 2.