Fig. 2.

Growth and cell survival responses in PKD1/3^−/− DT40 B cells. (A) Exponentially growing wild-type, PKD1/3^−/−/PKD3⁺ and PKD1^−/−3^−/− (washed out of doxycycline for at least 5 days) DT40 B cells were plated at a cell density of 1 × 10⁴/ml and cell number was counted daily. Results shown are the mean ± S.E. of triplicate cultures and are representative of two to three independent experiments. (B) Wild-type and PKD1/3^−/− DT40 B cells were cultured at a density of 1 × 10⁵ cells/ml with increasing concentrations of the indicated stimuli. After 24–48 h in culture, the number of viable cells in the cultures was determined by flow cytometry, using CalBRITE (Becton Dickinson) beads for quantitation of cell numbers. The data are expressed as a percentage of the number of viable cells present in control, non-treated cultures for each cell line (untreated = 100%). In some cases, the data are presented as the % cell death after 24 h as determined by flow cytometry, gating on 7AAD⁺ cells. All results are representative of at least two to four independent experiments. TSA, Trichostatin A.