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. 2007 Apr 3;581(7):1377–1382. doi: 10.1016/j.febslet.2007.02.055

Fig. 3.

Fig. 3

BCR-induced signalling events in PKD1/3−/− DT40 B cells. (A) Flow cytometric analysis of surface BCR expression (as detected by IgM levels) in wild-type, PKD1/3−/−/PKD3+ and PKD1/3−/− DT40 cells. (B) Wild-type and PKD1/3−/− DT40 cells were cultured in 0.5% FBS-containing media for 6 h, then with or without 10 μg/ml M4 (BCR) or 25 ng/ml PdBu (P) for the indicated times and cellular extracts were analysed by SDS–PAGE and Western blotting with the indicated antibodies. (C) Wild-type and PKD1/3−/− DT40 cells, pretreated for 1 h with 5 μM GF109203X (GF) or DMSO solvent (−) were stimulated with or without 25 ng/ml PdBu (P) or 10 μg/ml M4 (BCR) for 30 min before cellular extracts were analysed by SDS–PAGE and Western blotting with a phospho-LxRxxpS/T motif antibody (CST, #4381).