Fig. 4.

NFκB transcriptional activity in PKD1/3^−/− DT40 B cells. (A) The indicated DT40 B cell lines were transiently transfected with an NFκB-CAT reporter construct. The cells were then left untreated or were stimulated with either 10 μg/ml M4 (B) or 25 ng/ml PdBu (P) for 6 h before the activity of the reporter construct was analysed as described in Section 2. (B) Expression and activation of PKD enzymes in wild-type, PKD1/3^−/− and PKCβ^−/− DT40 B cells was performed as described in Fig. 1A.