Poly[U]-induced channel blockade lifetimes were
proportional to (a) mean polymer length and
(b) inversely proportional to applied voltage. The plots
show lifetimes for (a) peaks 1 (+), 2
(□), and 3 (•) in experiments using
V = −120 mV with 13 different size selected
poly[U]s and (b) for peaks 2 (□), and 3
(•) with poly[U] of mean length 215 nt at the indicated
voltages. Although the peak 1 lifetime appeared to be independent of
the applied voltage (data not shown), because this lifetime (≈100
μs) was barely a factor of 2 greater than the time resolution of our
system, a slight voltage dependence could not be ruled out.