Figure 6 Serum response factor (SRF) protein stability depends on integrin‐linked kinase (ILK) activation in Het1A cells. (A) Northern blot analyses showing that transforming growth factor β1 (TGFβ1) treatment upregulates SRF transcription regardless of ILK activity. (B) Western blot analysis showing the time course of SRF protein stability: cells were pretreated with TGFβ1 for 6 h, cycloheximide was then added at 100 μg/ml, and total protein was isolated at the times indicated. (C) Plot of SRF decay in ILK(−) cells versus controls: SRF protein content at each time point (SRFt) divided by SRF content at time 0 (SRF0) based on six replicates. *p<0.001. Error bars represent SD.