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. 1998 Mar 3;95(5):2586–2591. doi: 10.1073/pnas.95.5.2586

Figure 1.

Figure 1

Cloning of DENN/MADD and its splice variant. (A) Graphic representation of clone 1.1 and human DENN/MADD. Sequence comparison with C. elegans homolog AEX-3 also is illustrated. Shaded boxes are regions conserved between the two proteins. Three motifs in human are shown, one of which also occurs in AEX-3. (B) Northern analysis. A human tissue mRNA blot (CLONTECH) was used with clone 1.1 as the probe. Hybridization of the same blot with an actin cDNA probe indicated that similar amounts of RNA were present in each lane (data not shown). (C) Reverse transcription-PCR of DENN/MADD. mRNA (1 μg) from human brain was reverse-transcribed and amplified by using the primers illustrated in A: PCRI, nucleotides 2334–2689; PCRII, nucleotides 2334–3024. In both PCR reactions, multiple bands are seen. Clone 1.1 corresponds to the lower band in PCRII.