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Overexpression of TCP20 suppresses the actin organization defect of tor2ts cells. Logarithmic cultures of wild-type TOR2 (JK9-3da) (A and B) and tor2ts (SH121) cells carrying an empty vector (C and D) or pTCP20 (pAS27) (E and F) were grown at 30°C, shifted to 37°C for 6 h, fixed, stained with rhodamine phalloidin, and observed by fluorescence (A, C, and E) and Nomarski (B, D, and F) microscopy. tor2ts cells carrying an empty vector have a random actin distribution.
