Figure 3.
Effect of murine IP-10 expression on Burkitt lymphoma tumorigenesis. (A) Northern blot analysis of murine IP-10 mRNA expression in CA46-IP-10 clones 11/3, 11/4, and 11/9, vector control transfected and CA46 pCEP4 clones 1 and 3, and murine IP-10 expressing CHO cells. The RNA (15 mg) was run on a 1.2% agarose gel, transferred to nitrocellulose membrane, and hybridized for murine IP-10 and murine GAPDH. (B) Tumor growth in BALB/c nu/nu mice inoculated subcutaneously with murine IP-10-expressing and control CA46 Burkitt cells. Murine IP-10 expressing CA46 clones 11/3 (▪), 11/4 (▴), and 11/9 (•) and control CA46 clones 1 (□) and 3 (○) (107 cells in 0.2 ml culture medium), were each inoculated subcutaneously in irradiated (400 rad) BALB/c nu/nu mice. Tumor size was measured weekly. Each data point represents the mean tumor size of four or five mice. Because of large tumor size and mouse mobility impairment, mice injected with CA46-pCEP4 clones 1 and 3 were killed at 7 weeks.