Table 1.
Detection of Skb1–Shk1 and Skb1–Skb1 interactions in the two-hybrid system
| DNA-binding domain fusions | Activating domain
fusions
|
||||||
|---|---|---|---|---|---|---|---|
| Skb1 | Shk1 | Pak1 | Cdc42 | Ras1 | Scd1 | Scd2 | |
| LBD fusions | |||||||
| Shk1 | + | + | ND | + | − | − | − |
| Ste20 | − | ND | ND | + | − | − | − |
| Cdc42 | − | + | + | ND | − | − | + |
| Rac1 | − | + | + | ND | ND | ND | ND |
| Ras1 | − | − | − | − | ND | ND | ND |
| Gpa1 | − | − | ND | ND | − | ND | ND |
| Byr2 | − | − | ND | ND | + | ND | ND |
| Ste11 | − | − | ND | ND | − | ND | ND |
| Raf1 | − | − | ND | ND | + | ND | ND |
| Ste7 | − | − | ND | ND | − | ND | ND |
| Mek1 | − | ND | ND | ND | − | ND | ND |
| Scd1 | − | − | ND | ND | − | ND | + |
| GBD fusions | |||||||
| Skb1 | + | + | − | − | − | − | − |
| Shk1 | + | ND | ND | + | − | ND | ND |
| Gpa1 | − | − | ND | ND | − | ND | ND |
| Scd2 | − | − | ND | + | − | ND | ND |
| Byr1 | − | ND | ND | − | ND | ND | ND |
Values represent the presence of transformed colonies that expressed detectable β-gal activity (+) or not (−). ND, not determined. Activating domain fusions were to the activating domain of S. cerevisiae Gal4 (GAD). GBD–GAD combinations were expressed in the Gal4 two-hybrid tester strain, HF7c. LBD–GAD combinations were tested in the LexA tester strain, L40. At least eight independent transformants were tested for each determination.