Figure 1.

Strategy used in this study. (A) Motility genes were studied by screening 3985 E. coli mutants for motility defects (24 are shown in the top left panel) and by screening motility proteins for protein interactions (here: bait FlaB1 from T. pallidum returning preys FlaB1 and FliS). These data were integrated with literature interactions and large-scale interaction data sets to refine the current picture of the bacterial flagellum. (B) Comparison of motility genes identified in E. coli (this study) and B. subtilis (Schumann et al, 2001). There are 43 ‘known' motility genes reported in the literature for E. coli, 34 of which have orthologs in B. subtilis. Our mutant screens identified 116 additional ‘new' motility genes of which only 7 have orthologs in B. subtilis. Equivalent numbers are given for B. subtilis (individual genes are listed in Supplementary Tables S1 and S3).