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Effect of IR on RPA p34 hyperphosphorylation in MO59J cells demonstrated by immunoblot analysis. MO59K and MO59J human glioblastoma cells were given a 50-Gy dose of IR. Crude protein extracts were obtained at the times indicated after irradiation. Proteins were separated by 15% SDS/PAGE. Blots were probed with a polyclonal antibody specific for RPA p34 and immunoreactive bands were detected by ECL.
