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. 1998 Mar 3;95(5):2630–2635. doi: 10.1073/pnas.95.5.2630

Figure 1.

Figure 1

Targeted mutagenesis of the Tac1 gene. (A), Map of the wild-type genomic locus and cDNA from the mouse strains 129SV/J (Tac1129) and the C57BL/6J (Tac1C57), the targeting construct, and the recombinant locus (Tac1tTAneo). The DNA probe used for Southern blot analysis and the primers used for PCR analysis are indicated. H, HpaII; N, Nsi I; K, KpnI. (B) Southern blot analysis of a recombinant ES cell after digestion with Nsi I or KpnI. (C) Genotyping of offspring from Tac1tTAneo/C57 × Tac1tTAneo/C57 matings by PCR analysis using primer P1, P2, and P3. (D), Genotyping of offspring from Tac1129/C57 × Tac1129/C57 matings by PCR amplification with primers P4 and P5, and subsequent digestion with HpaII. Note that HpaII cuts only the PCR fragment derived from the 129 allele, but not the C57BL/6 allele. Homozygous mutant Tac1tTAneo/tTAneo and wild-type Tac1129/129 are referred to as Tac1−/− and Tac1+/+ mice, respectively, throughout the text.