Figure 1.

Alignment of GLH-1 and GLH-2. N-terminal glycine-rich imperfect repeats are shaded in grey. CCHC zinc fingers are indicated with black backing. The zinc finger consensus in the two GLH proteins is PxxCFNCxxxGHRSxxCPEP; these amino acids are found in at least 7/10 fingers. Comparisons of specific zinc fingers are shown at the bottom with differences in black. The conserved motifs found in all DEAD-box RNA helicases (26) are boxed. The 22-amino acid peptides used to produce GLH-1 and GLH-2-specific peptides are underlined (N termini). Sites of introns in the corresponding glh-1 and glh-2 genomic sequences are indicated with arrowheads. The 58 amino acids encoded by a 168-nt internal BamHI–BamHI fragment were absent in the original report of the glh-1 cDNA (18); the corrected sequence is GenBank accession no. L19948L19948.