Figure 5.

Immunofluorescence staining of adult gonads using anti-GLH-1 and anti-GLH-2 antibodies. Each row shows a sample stained with affinity-purified mouse anti-GLH-1 antibodies (Left), chicken anti-GLH-2 (Center), and DAPI (Right). Gonad arms are oriented with distal left. (A) Distal gonad arm from a wild-type hermaphrodite. (B) Gonad arm from a wild-type male. Sperm present at the far right of each panel fail to stain with anti-GLH-1 and anti-GLH-2. (C) Gonad arm from a sterile hermaphrodite worm produced by a mother injected with antisense RNA to glh-1. (Bar = 10 μm.)