Figure 1.

(A) Maps of plasmids and PCR primers used in this report. Arrows point in the direction of transcription of the wild-type PMCA1 gene. The approximate position of the initiation codon is indicated by “AUG,” which is at position 182 in the human PMCA1 mRNA. The fragment contains 181 bp of 5′-untranslated sequence and 265 bp of coding sequence. (B) Western blot of PMCA1 isoforms in PC6 cells and transfectants. Partially purified calmodulin-binding proteins from membrane preparations of PC6 cells and transfectants expressing PMCA1 antisense RNA (RSV9-2, RSV9-9) or sense RNA (RSV14-2, RSV14-4) was resolved on a 7.5% SDS/PAGE and transferred to an Immobilon membrane. The blot was probed with an antibody that recognizes all PMCA1 isoforms (designated PMCA1N; ref. 15).