Figure 3.

(A) NGF responsiveness of PC6 cells. Two PMCA1 antisense RNA expressing transfectants (RSV9-2, RSV9-9) and two PMCA1 sense RNA expressing control transfectants (RSV14-2, RSV14-4) were plated on standard tissue culture plastic and allowed to attach overnight. After attachment, fresh medium containing 100 ng/ml 2.5S murine NGF was added and the cells were allowed to continue in culture. The medium, with fresh NGF, was changed every 3 days. Cells were photographed on day 6. (B) Quantitation of neurite length in PMCA1 antisense expressing cells and controls. the length of neurites on cells in A was determined. Length was expressed in cell body diameters of the cell to which the neurite was attached. Length was only measured for neurites that remained in the frame of the picture and whose attachment to a cell could be clearly seen. For branched neurites only the longest branch was scored.